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Response of human bone marrow‐derived MSCs on triphasic Ca‐P substrate with various HA/TCP ratio
Author(s) -
Bajpai Indu,
Kim Duk Yeon,
KyongJin Jung,
Song InHwan,
Kim Sukyoung
Publication year - 2017
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33538
Subject(s) - calcium , fibronectin , extracellular matrix , in vitro , chemistry , materials science , cell growth , microbiology and biotechnology , biochemistry , biology , organic chemistry
Calcium phosphates (Ca‐P) are used commonly as artificial bone substitutes to control the biodegradation rate of an implant in the body fluid. This study examined the in vitro proliferation of human bone marrow‐derived mesenchymal stem cells (hBMSCs) on triphasic Ca‐P samples. For this aspect, hydroxyapatite (HA), dicalcium phosphate dehydrate (DCPD), and calcium hydroxide (Ca(OH) 2 ) were mixed at various ratios, cold compacted, and sintered at 1250°C in air. X‐ray diffraction showed that the β‐tricalcium phosphate (TCP) to α‐TCP phase transformation increased with increasing DCPD/HA ratio. The micro‐hardness deceased with increasing TCP content, whereas the mean grain size and porosity increased with increasing TCP concentration. To evaluate the in vitro degree of adhesion and proliferation on the HA/TCP samples, human BMSCs were incubated on the HA/TCP samples and analyzed by a cells proliferation assay, expression of the extracellular matrix (ECM) genes, such as α‐smooth muscle actin (α‐SMA) and fibronectin (FN), and FITC‐phalloidin fluorescent staining. In terms of the interactions of human BMSCs with the triphasic Ca‐P samples, H50T50 (Ca/P = 1.59) markedly enhanced cell spreading, proliferation, FN, and α‐SMA compared with H100T0 (Ca/P = 1.67). Interestingly, these results show that among the five HA/TCP samples, H50T50 is the optimal Ca‐P composition for in vitro cell proliferation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 72–80, 2017.