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Porous poly ( D,L ‐lactide‐ co ‐glycolide) acid/biosilicate ® composite scaffolds for bone tissue engineering
Author(s) -
Kido Hueliton W.,
Brassolatti Patricia,
Tim Carla R.,
GabbaiArmelin Paulo R.,
Magri Angela M.P.,
Fernandes Kelly R.,
Bossini Paulo S.,
Parizotto Nivaldo A.,
Crovace Murilo C.,
Malavazi Iran,
da Cunha Anderson F.,
Plepis Ana M.G.,
Anibal Fernanda F.,
Rennó Ana C.M.
Publication year - 2017
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33536
Subject(s) - plga , bone healing , glycolic acid , chemistry , bone morphogenetic protein 2 , runx2 , lactic acid , surgery , medicine , gene expression , biochemistry , biology , in vitro , gene , bacteria , genetics
This study evaluated the effects of the Biosilicate ® and poly (D,L‐lactic‐ co ‐glycolic) acid composites on bone repair in a tibial bone defect model in rats by means of using histological evaluation (histopathological and morphometric analysis) and gene expression analysis. Eighty male Wistar rats (12 weeks old, weighing ±300 g) were randomly divided into two groups: Biosilicate ® group (BG) and Biosilicate ® /PLGA group (BG/PLGA). Each group was euthanized at 3, 7, 14, and 21 days after surgery ( n = 10 animals per time point). The main findings showed that the incorporation of PLGA into BG had a significant effect on the morphological structure of the material, accelerating mass loss, decreasing the pH and increasing the calcium release. Furthermore, histologic analysis revealed that the BG/PLGA showed increased material degradation, accompanied by higher bone formation compared to BG, after 21 days of implantation. In addition, qRT‐PCR analysis showed that BG/PLGA induced an upregulation of the osteogenic genes related to BMP4, Runx2, ALP, and OC. These results show that the present BG/PLGA composite may be used as a bone graft for inducing bone repair. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 63–71, 2017.