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Point‐of‐care seeding of nitinol stents with blood‐derived endothelial cells
Author(s) -
Jantzen Alexandra E.,
Noviani Maria,
Mills James S.,
Baker Katherine M.,
Lin FuHsiung,
Truskey George A.,
Achneck Hardean E.
Publication year - 2016
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33510
Subject(s) - restenosis , stent , biomedical engineering , delivery system , in vivo , ex vivo , materials science , seeding , endothelium , surgery , medicine , biology , agronomy , microbiology and biotechnology
Nitinol‐based vascular devices, for example, peripheral and intracranial stents, are limited by thrombosis and restenosis. To ameliorate these complications, we developed a technology to promote vessel healing by rapidly seeding ( QuickSeeding ) autologous blood‐derived endothelial cells (ECs) onto modified self‐expanding nitinol stent delivery systems immediately before implantation. Several thousand micropores were laser‐drilled into a delivery system sheath surrounding a commercial nitinol stent to allow for exit of an infused cell suspension. As suspension medium flowed outward through the micropores, ECs flowed through the delivery system attaching to the stent surface. The QuickSeeded ECs adhered to and spread on the stent surface following 24‐h in vitro culture under static or flow conditions. Further, QuickSeeded ECs on stents that were deployed into porcine carotid arteries spread to endothelialize stent struts within 48 h ( n = 4). The QuickSeeded stent struts produced significantly more nitric oxide in ex vivo flow circuits after 24 h, as compared to static conditions ( n = 5). In conclusion, ECs QuickSeeded onto commercial nitinol stents within minutes of implantation spread to form a functional layer in vitro and in vivo , providing proof of concept that the novel QuickSeeding method with modified delivery systems can be used to seed functional autologous endothelium at the point of care. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1658–1665, 2016.