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Mechanical properties of human autologous tubular connective tissues (human biotubes) obtained from patients undergoing peritoneal dialysis
Author(s) -
Nakayama Yasuhide,
Kaneko Yoshiyuki,
Takewa Yoshiaki,
Okumura Noriko
Publication year - 2016
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33495
Subject(s) - beagle , connective tissue , catheter , medicine , biomedical engineering , peritoneal dialysis , anatomy , materials science , surgery , pathology
Completely autologous in vivo tissue‐engineered connective tissue tubes (Biotubes) have promise as arterial vascular grafts in animal implantation studies. In this clinical study of patients undergoing peritoneal dialysis (PD) ( n = 11; age: 39–83 years), we evaluated human Biotubes' (h‐Biotubes) mechanical properties to determine whether Biotubes with feasibility as vascular grafts could be formed in human bodies. We extracted PD catheters, embedded for 4–47 months, and obtained tubular connective tissues as h‐Biotubes (internal diameter: 5 mm) from around the catheter' silicone tubular parts. h‐Biotubes were composed mainly of collagen with smooth luminal surfaces. The average wall thickness was 278 ± 178 μm. No relationship was founded between the tubes' mechanical properties and patients' ages or PD catheter embedding periods statistically. However, the elastic modulus (2459 ± 970 kPa) and tensile strength (623 ± 314 g) of h‐Biotubes were more than twice as great as those from animal Biotubes, formed from the same PD catheters by embedding in the beagle subcutaneous pouches for 1 month, or beagle arteries. The burst strength (6338 ± 1106 mmHg) of h‐Biotubes was almost the same as that of the beagle thoracic or abdominal aorta. h‐Biotubes could be formed in humans over a 4‐month embedding period, and they satisfied the mechanical requirements for application as vascular grafts. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1431–1437, 2016.