Premium
Bottom‐up topography assembly into 3 D porous scaffold to mediate cell activities
Author(s) -
Cheng Delin,
Hou Jie,
Hao Lijing,
Cao Xiaodong,
Gao Huichang,
Fu Xiaoling,
Wang Yingjun
Publication year - 2016
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33452
Subject(s) - scaffold , extracellular matrix , mesenchymal stem cell , cell adhesion , microbiology and biotechnology , adhesion , plga , materials science , matrix (chemical analysis) , integrin , cell , tissue engineering , chemistry , biomedical engineering , biophysics , in vitro , biology , biochemistry , composite material , medicine
Native cells live in a three‐dimensional (3D) extracellular matrix (ECM) capable of regulating cell activities through various physical and chemical factors. Designed topographies have been well proven to trigger significant difference in cell behaviours. However, present topographies are almost all constructed on two‐dimensional (2D) substrates like discs and films, which are far from features like 3D and porosity required in application like bone repair. Here we bottom‐up assembled poly(lactic‐ co ‐glycolic acid)/calcium carbonate (PLGA/CC) microspheres with superficial porous topography intactly into a 3D porous scaffold. Because the scaffold was obtained through a mild technique, the bioactivity of released BMP‐2 was well retained. Mouse bone marrow mesenchymal stem cells (mMSCs) were cultured on produced scaffolds having different 3D topographies. It turned out that osteogenic differentiation of mMSCs did respond to the 3D topographies, while proliferation didn't. Gene expression of α v and β 1 integrins revealed that adhesion was supposed to be the underlying mechanism for osteogenic response. The study provides insight into enhancing function of practical scaffolds by elaborate topography design. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1056–1063, 2016.