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Feasibility of a nanomaterial‐tissue patch for vascular and cardiac reconstruction
Author(s) -
Ostdiek Allison M.,
Ivey Jan R.,
Hansen Sarah A.,
Gopaldas Raja,
Grant Sheila A.
Publication year - 2016
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33410
Subject(s) - medicine , histology , decellularization , angioplasty , in vivo , pericardium , stenosis , cd31 , trichrome , intravascular ultrasound , masson's trichrome stain , pathology , anatomy , tissue engineering , biomedical engineering , fibrosis , surgery , cardiology , immunohistochemistry , biology , h&e stain , microbiology and biotechnology
Vascular and cardiac reconstruction involves the use of biological patches to treat trauma and defects. An in vivo study was performed to determine the remodeling and biologic effects of novel nanostructured vascular patches with and without gold nanoparticles. Porcine vascular tissue was decellularized and conjugated with gold nanoparticles to evaluate if integration would occur while avoiding rupture and stenosis. Swine underwent a bilateral patch angioplasty of the carotid arteries with experimental patches on the right and control patches of bovine pericardium on the left. Animals were sacrificed after surgery and at 3 and 9 weeks. Ultrasound was performed during surgery, every 3 weeks, and before euthanasia. Endothelial regeneration was examined using Evans Blue dye and histology using Trichrome and H&E. There was a 100% success rate of implantation with 0% mortality. All patches were patent on ultrasound. At 3 weeks, experimental patches had regenerating endothelial cell growth and normal healing responses. At 9 weeks, the experimental patches demonstrated excellent integration. Histology demonstrated cellular in‐growth into the experimental patches and no major immune reactions. This is one of the first studies to demonstrate the feasibility of nanomaterial‐tissue patches for vascular and cardiac reconstruction. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 449–457, 2016.