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Immunolocalization of dentin matrix protein‐1 in human primary teeth treated with different pulp capping materials
Author(s) -
Lourenço Neto Natalino,
Marques Nádia C. T.,
Fernandes Ana Paula,
Rodini Camila O.,
Sakai Vivien T.,
Abdo Ruy Cesar C.,
Machado Maria Aparecida A. M.,
Santos Carlos F.,
Oliveira Thais M.
Publication year - 2016
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33379
Subject(s) - mineral trioxide aggregate , pulpotomy , pulp capping , dentin , dentistry , pulp (tooth) , resorption , immunostaining , molar , odontoblast , calcium hydroxide , calcification , chemistry , materials science , immunohistochemistry , medicine , pathology
Abstract The aim of this study was to evaluate the immunolocalization of dentin matrix protein (DMP)‐1 in human primary teeth treated with different pulp capping materials. Twenty‐five primary molars were divided into the following groups: formocresol (FC), calcium hydroxide (CH), mineral trioxide aggregate (MTA), corticosteroid/antibiotic solution + CH (O + CH), and Portland cement (PC), and all received conventional pulpotomy treatment. The teeth at the regular exfoliation period were extracted for histological analysis and immunolocalization of DMP‐1. Statistical analysis was performed using the χ 2 test ( p < 0.05). Histological analysis revealed statistically significant differences in the comparison among the groups through the use of a score system regarding the presence of hard tissue barrier, odontoblastic layer, and internal resorption, but not regarding pulp calcification. Immunohistochemical analysis showed immunostaining for DMP‐1 in groups CH, MTA, O + CH, and PC. Internal resorption was observed in the groups FC and CH. MTA and PC showed pulp repair without inflammation and with the presence of hard tissue barrier. DMP‐1 immunostaining was higher for MTA and PC, confirming the reparative and bioinductive capacity of these materials. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 165–169, 2016.