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Investigation of the cytotoxicity of aluminum oxide nanoparticles and nanowires and their localization in L 929 fibroblasts and RAW 264 macrophages
Author(s) -
Hashimoto Masanori,
Sasaki JunIchi,
Imazato Satoshi
Publication year - 2016
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33377
Subject(s) - transmission electron microscopy , cytotoxicity , viability assay , fluorescence microscope , scanning electron microscope , vesicle , biophysics , materials science , nanotechnology , immunostaining , nanoparticle , electron microscope , nanomaterials , chemistry , genotoxicity , cell , fluorescence , in vitro , biochemistry , biology , membrane , toxicity , immunology , organic chemistry , composite material , immunohistochemistry , physics , optics , quantum mechanics
The biological responses of aluminum oxide (Al 2 O 3 ) nanoparticles (NPs) and nanowires (NWs) in cultured fibroblasts (L929) and macrophages (RAW264) were evaluated from their cytotoxicities and micromorphologic properties. Cultured cells were exposed to Al 2 O 3 NPs (13 nm diameter) and Al 2 O 3 NWs (2–6 × 200–400 nm). Cytotoxicity and genotoxicity were examined by immunostaining with fluorescence microscopy, and nanomaterial localization was studied by using scanning electron microscopy and transmission electron microscopy. The NPs were cytotoxic and genotoxic, whereas the NWs were not. The scanning electron microscopy images showed that the NPs aggregate more on the cell surface than do the NWs. The transmission electron microscopy images showed that the NPs were internalized into the vesicle and nuclei, for both cell types. In contrast, numerous solid NWs were observed as large aggregates in vesicles, but not in nuclei. Nuclear damage was confirmed by measuring cell viability and by immunostaining for NPs. The chemical changes induced by the NPs in the vesicles or cells may cause cell damage because of their large surface area per volume. The extent of NW entrapment was not sufficient to lower the viability of either cell type. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 241–252, 2016.