Premium
Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
Author(s) -
Balmayor Elizabeth R.,
Baran Turker E.,
Unger Marina,
Marques Alexandra P.,
Azevedo Helena S.,
Reis Rui L.
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33343
Subject(s) - chitosan , starch , chemistry , biocompatibility , permeability (electromagnetism) , gentamicin , biodegradation , drug delivery , chemical engineering , biophysics , nuclear chemistry , biochemistry , organic chemistry , antibiotics , membrane , engineering , biology
The effect of α‐amylase degradation on the release of gentamicin from starch‐conjugated chitosan microparticles was investigated up to 60 days. Scanning electron microscopic observations showed an increase in the porosity and surface roughness of the microparticles as well as reduced diameters. This was confirmed by 67% weight loss of the microparticles in the presence of α‐amylase. Over time, a highly porous matrix was obtained leading to increased permeability and increased water uptake with possible diffusion of gentamicin. Indeed, a faster release of gentamicin was observed with α‐amylase. Starch‐conjugated chitosan particles are non‐toxic and highly biocompatible for an osteoblast (SaOs‐2) and fibroblast (L929) cell line as well as adipose‐derived stem cells. When differently produced starch‐conjugated chitosan particles were tested, their cytotoxic effect on SaOs‐2 cells was found to be dependent on the crosslinking agent and on the amount of starch used. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1610–1620, 2015.