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Novel tea polyphenol‐modified calcium phosphate nanoparticle and its remineralization potential
Author(s) -
He Libang,
Deng Dongmei,
Zhou Xuedong,
Cheng Lei,
Cate Jacob M.,
Li Jiyao,
Li Xudong,
Crielaard Wim
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33333
Subject(s) - remineralisation , nuclear chemistry , amorphous calcium phosphate , phosphate , chemistry , calcium , nanoparticle , scanning electron microscope , antioxidant , polyphenol , enamel paint , chemical engineering , inorganic chemistry , biochemistry , materials science , fluoride , nanotechnology , organic chemistry , engineering , composite material
Tea polyphenols (TP) are not only potent antimicrobial and antioxidant agents but also effective modifiers in the formation of nanosized crystals. Since nano‐hydroxyapatite (n‐HA) is known to enhance remineralization of dental hard tissue, our aims were to synthesize nanosized calcium phosphate particles incorporating TP and to test their potential as caries preventive agent. An ammonia water diffusion method was used to synthesize nanosized calcium phosphate particles (TP‐CaP) in the presence of various amounts of TP. The resultant products were characterized by scanning electron microscopy (SEM) and X‐ray diffraction (XRD). The remineralization potential of the nano TP‐CaP was then investigated in a 12‐day pH‐cycling model. Nano TP‐CaP slurries, at pH 7.0 and pH 5.5, were applied onto preformed enamel lesions 4 × 3 min per day. n‐HA slurries at pH 7.0 and pH 5.5 were used as positive controls, and deionized water was served as a negative control. SEM showed nanosized particles were only formed at 27 mg/mL of TP. Further characterization of the nanosized particles revealed the components were amorphous calcium phosphate, HA, and TP. Both surface microhardness and transverse microradiography analyses showed that nano TP‐CaP at pH 5.5, but not at pH 7.0, significantly enhanced remineralization, to the same extent as the n‐HA controls. Furthermore, significantly higher amount of TP was found in the supernatant of TP‐CaP at pH 5.5 than those at pH 7.0. Since TP can inhibit bacterial growth and enzyme activities, the novel nanosized TP‐CaP particle, at low pH, is a potential dual‐functional‐remineralization and antibacteria‐product. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1525–1531, 2015.