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Electrospun poly(ε‐caprolactone)‐based skin substitutes: I n vivo evaluation of wound healing and the mechanism of cell proliferation
Author(s) -
Augustine Robin,
Dominic Edwin Anto,
Reju Indu,
Kaimal Balarama,
Kalarikkal Nandakumar,
Thomas Sabu
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33325
Subject(s) - wound healing , in vivo , cell growth , microbiology and biotechnology , fibroblast , chemistry , cell , cell migration , caprolactone , cell adhesion , in vitro , immunology , medicine , biology , biochemistry , polymer , polymerization , organic chemistry
Abstract In the present study, we have fabricated electrospun poly(ε‐caprolactone)‐based membranes, characterized and studied the in vivo cell migration and proliferation and wound healing activity. Moreover, we did not seed any cells prior to the animal implantation and we could observe excellent fibroblast attachment and cell proliferation. Further full thickness excision wound on guinea pig completely healed within 35 days. We could reach in an assumption that the enhanced cell proliferation and wound healing might be due to the surface degradation of the polymer under physiological conditions and the formation of functional groups like hydroxyl and carboxyl groups that promoted cell proliferation in a cell adhesion protein mediated mechanism. This study is a novel tissue engineering concept for the reconstruction of a damaged tissue without the in vitro cell seeding and proliferation prior to the in vivo implantation. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1445–1454, 2015.