Premium
Fabrication of poly HEMA grids by micromolding in capillaries for cell patterning and single‐cell arrays
Author(s) -
Ye Fang,
Ma Binghe,
Gao Jie,
Xie Li,
Wei Chen,
Jiang Jin
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33300
Subject(s) - polydimethylsiloxane , microfabrication , pdms stamp , methacrylate , materials science , fabrication , microfluidics , nanotechnology , tissue engineering , biomaterial , adhesive , surface modification , chemistry , biomedical engineering , polymer , composite material , polymerization , layer (electronics) , medicine , alternative medicine , pathology
Control of cell adhesion and growth by microfabrication technology and surface chemistry is important in an increasing number of applications in biotechnology and medicine. In this study, we developed a method to fabricate (2‐hydroxyethyl methacrylate) (polyHEMA) grids on glass by micromolding in capillaries (MIMIC). As a non‐fouling biomaterial, polyHEMA was used to inhibit the nonspecific bonding of cells, whereas the glass surface provided a cell adhesive background. The polyHEMA chemical barrier was directly obtained using MIMIC without surface modification, and the microchannel networks used for capillarity were easily achieved by reversibly bonding the polydimethylsiloxane (PDMS)mold and the glass. After fabrication of the polyHEMA micropattern, individual cytophilic microwells surrounded by cytophobic sidewalls were presented on the glass surface. The polyHEMA micropattern proved effective in controlling the shape and spreading of cells, and square‐shaped mouse osteoblast MC3T3‐E1 cells were obtained in microwell arrays after incubation for 3 days. Moreover, the widths of the microwells in this micropattern were optimized for use as single‐cell arrays. The proposed method could be a convenient tool in the field of drug screening, stem cell research, and tissue engineering. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1375–1380, 2015.