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The heat‐compression technique for the conversion of platelet‐rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation
Author(s) -
Kawase Tomoyuki,
Kamiya Mana,
Kobayashi Mito,
Tanaka Takaaki,
Okuda Kazuhiro,
Wolff Larry F.,
Yoshie Hiromasa
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33262
Subject(s) - platelet rich fibrin , fibrin , membrane , biomedical engineering , biocompatibility , materials science , in vivo , plasmin , regeneration (biology) , chemistry , barrier membrane , tissue engineering , biochemistry , medicine , microbiology and biotechnology , biology , immunology , metallurgy , enzyme
Abstract Platelet‐rich fibrin (PRF) was developed as an advanced form of platelet‐rich plasma to eliminate xenofactors, such as bovine thrombin, and it is mainly used as a source of growth factor for tissue regeneration. Furthermore, although a minor application, PRF in a compressed membrane‐like form has also been used as a substitute for commercially available barrier membranes in guided‐tissue regeneration (GTR) treatment. However, the PRF membrane is resorbed within 2 weeks or less at implantation sites; therefore, it can barely maintain sufficient space for bone regeneration. In this study, we developed and optimized a heat‐compression technique and tested the feasibility of the resulting PRF membrane. Freshly prepared human PRF was first compressed with dry gauze and subsequently with a hot iron. Biodegradability was microscopically examined in vitro by treatment with plasmin at 37°C or in vivo by subcutaneous implantation in nude mice. Compared with the control gauze‐compressed PRF, the heat‐compressed PRF appeared plasmin‐resistant and remained stable for longer than 10 days in vitro . Additionally, in animal implantation studies, the heat‐compressed PRF was observed at least for 3 weeks postimplantation in vivo whereas the control PRF was completely resorbed within 2 weeks. Therefore, these findings suggest that the heat‐compression technique reduces the rate of biodegradation of the PRF membrane without sacrificing its biocompatibility and that the heat‐compressed PRF membrane easily could be prepared at chair‐side and applied as a barrier membrane in the GTR treatment. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 825–831, 2015.