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The (PrS/HGF‐pDNA) multilayer films for gene‐eluting stent coating: Gene‐protecting, anticoagulation, antibacterial properties, and in vivo antirestenosis evaluation
Author(s) -
Chang Hao,
Ren Kefeng,
Zhang He,
Wang Jinlei,
Wang Bailiang,
Ji Jian
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33224
Subject(s) - in vivo , stent , biomedical engineering , coating , restenosis , materials science , gene delivery , hepatocyte growth factor , protamine sulfate , genetic enhancement , microbiology and biotechnology , cancer research , gene , nanotechnology , protamine , chemistry , medicine , biology , surgery , heparin , biochemistry , receptor
Abstract Vascular gene‐eluting stents (GES) is a promising strategy for treatment of cardiovascular disease. Very recently, we have proved that the (protamine sulfate/plasmid DNA encoding hepatocyte growth factor) (PrS/HGF‐pDNA) multilayer can serve as a powerful tool for enhancing competitiveness of endothelial cell over smooth muscle cell, which opens perspectives for the regulation of intercellular competitiveness in the field of interventional therapy. However, before the gene multilayer films could be used in vascular stents for real clinical application, the preservation of gene bioactivity during the industrial sterilization and the hemocompatibility of film should be taken into account. Actually, both are long been ignored issues in the field of gene coating for GES. In this study, we demonstrate that the (PrS/HGF‐pDNA) multilayer film exhibits the good gene‐protecting abilities, which is confirmed by using the industrial sterilizations (gamma irradiation and ethylene oxide) and a routine storage condition (dry state at 4°C for 30 days). Furthermore, hemocompatible measurements (such as platelet adhesion and whole blood coagulation) and antibacterial assays (bacteria adhesion and growth inhibition) indicate the good anticoagulation and antibacterial properties of the (PrS/HGF‐pDNA) multilayer film. The in vivo preliminary data of angiography and histological analysis suggest that the (PrS/HGF‐pDNA) multilayer coated stent can reduce the in‐stent restenosis. This work reveals that the (PrS/HGF‐pDNA) multilayer film could be a promising candidate as coating for GES, which is of great potential in future clinic application. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 430–439, 2015.