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The effect and safety of polylactic acid and adipose‐derived stromal vascular fraction cell as an injectable bulking agent in urologic field: A 24‐week follow‐up study
Author(s) -
Lee Seong Ho,
Ko Kyungtae,
Choo Min Soo,
Lee Won Ki,
Jeong Hyun Cheol,
Cho Sung Tae,
Kim Sung Yong,
Kim Hayoung,
Kang Won Hwa,
Kim Gun Poong,
Yang Dae Yul
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33221
Subject(s) - polylactic acid , stromal vascular fraction , microsphere , stromal cell , adipose tissue , medicine , chemistry , pathology , urology , biomedical engineering , chemical engineering , engineering , organic chemistry , polymer
Abstract The aim of this study is to evaluate whether polylactic acid (PLA) microspheres and adipose‐derived stromal vascular fraction (SVF) cells have appropriate properties as an injectable bulking agent in urologic field. Forty male Sprague–Dawley rats (2‐week‐old) were randomized into two groups. A total of 0.05 mL of PLA microsphere suspension and 0.05 mL of PLA microsphere suspension mixed with PKH26‐labeled SVF cells were injected into bladder wall in group I and group II, respectively. At 2, 8, 16, and 24 weeks of PLA microspheres injection, the volumes of implants were measured and bladder tissues including implants were analyzed and compared grossly and histologically between groups. The distant organs were examined histologically to determine migration of PLA microspheres. At 24 weeks of implantation, 65–70% of injected volume was maintained and there was no significant difference between groups. In histological analyses, injected PLA microspheres were localized in muscular layer of bladder without infiltration into adjacent layer. From 8 to 16 weeks of injection, hybrid tissues contained collagen and actin were observed between PLA microspheres and these findings were more clear in group II. PHK26‐labeled SVF cells were identified by fluorescence microscopy at all time points. There was no migration of PLA microspheres to other organs and no abnormality in weight gain and hematologic values. These results suggest the possibility of PLA microspheres as a potentially useful bulking agent in urologic field. And further investigation is needed to know synergic effect of SVF cells. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 440–447, 2015.