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In vitro investigation of antioxidant, anti‐Inflammatory, and antiplatelet adhesion properties of genistein‐modified poly(ethersulfone)/poly(vinylpyrrolidone) hemodialysis membranes
Author(s) -
Chang Teng,
DeFine Linda,
Alexander Thomas,
Kyu Thein
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33215
Subject(s) - genistein , membrane , adhesion , chemistry , in vitro , antioxidant , reactive oxygen species , cell adhesion , platelet , biophysics , materials science , biochemistry , immunology , medicine , organic chemistry , biology
Hemocompatibility of genistein‐modified poly(ethersulfone)/poly(vinylpyrrolidone) (PES/PVP) hemodialysis (HD) membranes has been investigated in vitro with emphasis on evaluation of cell viability, antioxidant, anti‐inflammatory, and antiplatelet adhesion properties. Genistein modified PES/PVP membranes reveal significant reduction of the reactive oxygen species and also considerable suppression of interleukin‐1β and tumor necrosis factor‐α levels in whole blood, but to a lesser extent ininterleukin‐6. The incorporation of PVP into the HD membrane reduces platelet adhesion by virtue of its hydrophilicity. Of particular importance is that platelet adhesion of the genistein modified membranes declines noticeably at low concentrations of genistein for about 5–10%, beyond which it raises the number of adhered platelets. The initial decline in the platelet adhesion is attributable to genistein's ability to inhibit intercellular and/or vascular cell adhesion, whereas the reversal of this adhesion trend with further increase of genistein loading is ascribed to the inherent hydrophobicity of the genistein modified HD membrane. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 539–547, 2015.