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In vitro degradation, hemolysis, and cytocompatibility of PEO/PLLA composite coating on biodegradable AZ31 alloy
Author(s) -
Wei Zhongling,
Tian Peng,
Liu Xuanyong,
Zhou Bangxin
Publication year - 2015
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33208
Subject(s) - plasma electrolytic oxidation , materials science , corrosion , coating , composite number , simulated body fluid , biocompatibility , alloy , magnesium alloy , microstructure , polylactic acid , composite material , electrolyte , chemical engineering , metallurgy , polymer , chemistry , scanning electron microscope , electrode , engineering
Magnesium and its alloys have large potential as degradable and absorbable biomaterials because of their mechanical properties and biocompatibility. However, their corrosion resistance is usually inadequate especially in physiological environment, which limits their broad applications in biomedical areas. In this work, plasma electrolytic oxidized/poly( l ‐lactide) (PEO/PLLA) composite coating was successfully fabricated on biodegradable AZ31 alloy by combing PEO process and sealing with PLLA. The microstructure, elemental composition, and phase composition of the PEO/PLLA composite coating were investigated. The in vitro degradation of the PEO/PLLA composite coating in simulated body fluid (SBF) was also systematically evaluated. The results revealed that the PEO/PLLA composite coating improved the corrosion resistance of AZ31 alloy significantly. The corrosion potential shifted from −1.663V to more positive position −1.317 V and the corrosion current density was reduced with six‐order of magnitude. The Mg 2+ ions, hydrogen release, and pH value change of solution caused by degradation were all decreased significantly. Moreover, the PEO process played a critical role in sustaining the integrity of the implant in long‐term service. The result of hemolysis test showed that the PEO/PLLA composite coating vested AZ31 alloy a low hemolysis ratio (0.806 ± 0.771)%, which is much lower than the safe value of 5% according to ISO 10993‐4. For the cytocompatibility test, compared with bare AZ31 alloy and PEO coating, MC3T3‐E1 cells showed much better adhesion and proliferation on the PEO/PLLA composite coating with nearly 4‐fold increase of cells after 7‐day cultivation, indicating that the PEO/PLLA composite coating has good biocompatibility for biomedical applications. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 342–354, 2015.