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Highly aligned porous Ti scaffold coated with bone morphogenetic protein‐loaded silica/chitosan hybrid for enhanced bone regeneration
Author(s) -
Jung HyunDo,
Yook SeWon,
Han CheolMin,
Jang TaeSik,
Kim HyounEe,
Koh YoungHag,
Estrin Yuri
Publication year - 2014
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33072
Subject(s) - scaffold , chitosan , bone morphogenetic protein 2 , materials science , regeneration (biology) , porosity , bone morphogenetic protein , biomedical engineering , chemical engineering , chemistry , composite material , microbiology and biotechnology , biochemistry , biology , engineering , in vitro , gene
Porous Ti has been widely investigated for orthopedic and dental applications on account of their ability to promote implant fixation via bone ingrowth into pores. In this study, highly aligned porous Ti scaffolds coated with a bone morphogenetic protein (BMP)‐loaded silica/chitosan hybrid were produced, and their bone regeneration ability was evaluated by in vivo animal experiments. Reverse freeze casting allowed for the creation of highly aligned pores, resulting in a high compressive strength of 254 ± 21 MPa of the scaffolds at a porosity level of ∼51 vol %. In addition, a BMP‐loaded silica/chitosan hybrid coating layer with a thickness of ∼1 μm was uniformly deposited on the porous Ti scaffold, which enabled the sustained release of the BMP over a prolonged period of time up to 26 days. The cumulative amount of the BMP released was ∼4 μg, which was much higher than that released from the specimen without a hybrid coating layer. In addition, the bone regeneration ability of the porous Ti scaffold with a BMP‐loaded silica/chitosan coating layer was examined by in vivo animal testing using a rabbit calvarial defect model and compared with those of the as‐produced porous Ti scaffold and porous Ti scaffold with a silica/chitosan coating layer. After 4 weeks of healing, the specimen coated with a BMP‐loaded silica/chitosan hybrid showed a much higher bone regeneration volume (∼36%) than the as‐produced specimen (∼15%) ( p < 0.005) and even the specimen coated with a silica/chitosan hybrid (∼25%) ( p < 0.05). © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 913–921, 2014.