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The beneficial effect of encapsulated human adipose‐derived stem cells in alginate hydrogel on neural differentiation
Author(s) -
Khosravizadeh Zahra,
Razavi Shahnaz,
Bahramian Hamid,
Kazemi Mohammad
Publication year - 2014
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33055
Subject(s) - nestin , neural stem cell , adipose tissue , viability assay , materials science , tissue engineering , stem cell , mtt assay , microbiology and biotechnology , neurosphere , biomedical engineering , neural tissue engineering , cell , cellular differentiation , adult stem cell , biology , biochemistry , medicine , gene
Tissue engineering employs combination of biomaterials and cell therapy to develop new therapeutic strategies for neurodegenerative diseases, spinal cord, and traumatic brain injuries. Alginate is a biocompatible hydrogel, which has been used broadly to encapsulate many types of cells. Human adipose‐derived stem cells (hADSCs) have appropriate property to differentiate into neuron‐like cells. Therefore, the aim of this study was to evaluate the effect of alginate hydrogel on the viability and neural differentiation potential of induced hADSCs. After neural induction of isolated hADSCs and encapsulated in alginate hydrogel, the cell viability using MTT assay and their neural differentiation potential by immunocytochemical and real time RT‐PCR analysis for neural markers (Nestin, GFAP, and MAP2) were evaluated. Expression of Nestin, GFAP, and MAP2 markers was significantly increased compare to monolayer induced cells ( p < 0.001), but we did not found any significant effect on viability of induced cells relative to monolayer induced cells. Although neural differentiation of encapsulated cells was increased relative to monolayer induced cells, the viability of these cells was not significantly different in alginate hydrogel as compared with monolayer induced cells. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 749–755, 2014.