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Screening study on hemolysis suppression effect of an alternative plasticizer for the development of a novel blood container made of polyvinyl chloride
Author(s) -
Haishima Yuji,
Kawakami Tsuyoshi,
Hasegawa Chie,
Tanoue Akito,
Yuba Toshiyasu,
Isama Kazuo,
Matsuoka Atsuko,
Niimi Shingo
Publication year - 2014
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33052
Subject(s) - plasticizer , polyvinyl chloride , hemolysis , container (type theory) , chemistry , polyvinyl alcohol , materials science , composite material , medicine
The aim of this study is to identify a plasticizer that is effective in the suppression of the autohemolysis of the stored blood and can be used to replace di(2‐ethylhexyl) phthalate (DEHP) in blood containers. The results of hemolysis test using mannitol‐adenine‐phosphate/red cell concentrates (MAP/RCC) spiked with plasticizers included phthalate, phthalate‐like, trimeliate, citrate, and adipate derivatives revealed that di‐isononyl‐cyclohexane‐1,2‐dicarboxylate (Hexamoll ® DINCH), di(2‐ethylhexyl)‐1,2,3,6‐tetrahydro‐phthalate (DOTP), and diisodecyl phthalate (DIDP) exhibited a hemolysis suppression effect almost equal to that of DEHP, but not other plasticizers. This finding suggested that the presence of 2 carboxy‐ester groups at the ortho position on a 6‐membered ring of carbon atoms may be required to exhibit such an effect. The hemolytic ratios of MAP/RCC‐soaked polyvinyl chloride (PVC) sheets containing DEHP or different amounts of DINCH or DOTP were reduced to 10.9%, 9.2–12.4%, and 5.2–7.8%, respectively (MAP/RCC alone, 28.2%) after 10 weeks of incubation. The amount of plasticizer eluted from the PVC sheet was 53.1, 26.1–36.5, and 78.4–150 µg/mL for DEHP, DINCH, and DOTP, respectively. PVC sheets spiked with DIDP did not suppress the hemolysis induced by MAP/RCC because of low leachability (4.8–6.0 µg/mL). These results suggested that a specific structure of the plasticizer and the concentrations of least more than ∼10 µg/mL were required to suppress hemolysis due to MAP/RCC. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 102B: 721–728, 2014.