Premium
The effect of sterilization methods on electronspun poly(lactide‐ co ‐glycolide) and subsequent adhesion efficiency of mesenchymal stem cells
Author(s) -
Braghirolli Daikelly Iglesias,
Steffens Daniela,
Quintiliano Kerlin,
Acasigua Gerson Arisoly Xavier,
Gamba Douglas,
Fleck Roland A.,
Petzhold Cesar L.,
Pranke Patricia
Publication year - 2014
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33049
Subject(s) - sterilization (economics) , materials science , nanofiber , mesenchymal stem cell , plga , electrospinning , biomedical engineering , biomaterial , adhesion , tissue engineering , scaffold , nanoparticle , nanotechnology , polymer , composite material , medicine , biology , microbiology and biotechnology , monetary economics , economics , foreign exchange market , foreign exchange
Abstract The sterilization of scaffolds is an essential step for tissue engineering in vitro and, mainly, clinical biomaterial use. However, this process can cause changes in the structure and surface of the scaffolds. Therefore, the objective of this study was to investigate the effect of sterilization by ethanol, ultraviolet radiation (UVR) or antimicrobial solution (AMS) on poly(lactide‐ co ‐glycolide) (PLGA) scaffolds produced by the electrospinning technique. The properties of nanofibers and the cellular adhesion of mesenchymal stem cells to the scaffolds were analyzed after the treatments. All methods generated sterile scaffolds but showed some kind of damage to the scaffolds. Ethanol and AMS caused changes in the morphology and scaffold dimensions, which were not observed when using the UVR method. However, UVR caused a greater reduction in polymeric molecular weight, which increased proportionally with exposure time of treatment. Nanofibers sterilized with AMS for 1 h and 2 h showed greater cellular adhesion than the other methods, demonstrating their potential as a method for sterilizing PLGA nanofibers. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 700–708, 2014.