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Laser fabrication of porous silicon‐based platforms for cell culturing
Author(s) -
Peláez RamónJ.,
Afonso CarmenN.,
Vega Fidel,
RecioSánchez Gonzalo,
TorresCosta Vicente,
MansoSilván Miguel,
GarcíaRuiz JosefaP.,
MartínPalma RaúlJ.
Publication year - 2013
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32966
Subject(s) - materials science , fabrication , silicon , porous silicon , laser , porosity , nanotechnology , optoelectronics , optics , composite material , medicine , alternative medicine , physics , pathology
In this study, we explore the selective culturing of human mesenchymal stem cells (hMSCs) on Si‐based diffractive platforms. We demonstrate a single‐step and flexible method for producing platforms on nanostructured porous silicon (nanoPS) based on the use of single pulses of an excimer laser to expose phase masks. The resulting patterns are typically 1D patterns formed by fringes or 2D patterns formed by circles. They are formed by alternate regions of almost unmodified nanoPS and regions where the nanoPS surface has melted and transformed into Si nanoparticles. The patterns are produced in relatively large areas (a few square millimeters) and can have a wide range of periodicities and aspect ratios. Direct binding, that is, with no previous functionalization of the pattern, alignment, and active polarization of hMSCs are explored. The results show the preferential direct binding of the hMSCs along the transformed regions whenever their width compares with the dimensions of the cells and they escape from patterns for smaller widths suggesting that the selectivity can be tailored through the pattern period. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 101B: 1463–1468, 2013.

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