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The effect of ethylene oxide sterilization on the surface chemistry and in vitro cytotoxicity of several kinds of chitosan
Author(s) -
França Rodrigo,
Mbeh Doris A.,
Samani Taraneh Djavanbakht,
Tien Canh,
Mateescu Mircea A.,
Yahia L'Hocine,
Sacher Edward
Publication year - 2013
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32964
Subject(s) - chitosan , sterilization (economics) , biocompatibility , chemistry , nuclear chemistry , fourier transform infrared spectroscopy , ethylene oxide , x ray photoelectron spectroscopy , lactate dehydrogenase , contact angle , polymer chemistry , chemical engineering , organic chemistry , polymer , copolymer , monetary economics , economics , foreign exchange market , enzyme , engineering , foreign exchange
The surfaces of three chitosan samples, differing only in their degrees of deacetylation and of carboxyethyl chitosan were chemically characterized by X‐ray photoelectron spectroscopy, time‐of‐flight secondary ion mass spectroscopy, X‐ray diffraction, and Fourier transform infrared, both before and after sterilization with ethylene oxide. Unexpected elemental ratios suggest that surface chemical modification occurred during the processing of the original chitin, with further surface modification on subsequent sterilization, despite previous reports to the contrary. Cell viability was evaluated by direct contact methyl thiazole tetrazolium and lactate dehydrogenase assays between the chitosan particles and A549 human epithelial cells, which demonstrated that the modifications incurred on sterilization are reflected in biocompatibility changes. All the samples were found to be biocompatible and nontoxic before sterilization and remained so subsequently. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 101B: 1444–1455, 2013.