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Effects of irradiation on osteoblast‐like cells on different titanium surfaces in vitro
Author(s) -
Li Yumei,
Ma Wei,
Feng Zhihong,
Wang Zhijun,
Zha Nianbao,
Deng Banglian,
Zhao Yimin
Publication year - 2013
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32803
Subject(s) - adhesion , titanium , osteoblast , cell adhesion , irradiation , in vitro , secretion , cell growth , chemistry , surface modification , cell , materials science , biophysics , microbiology and biotechnology , biochemistry , biology , physics , organic chemistry , nuclear physics
The aim of this study was to investigate the effects of irradiation on adhesion ability, proliferation, and differentiation of MC3T3‐E1 cells on microarc oxidation (MAO) titanium surfaces and polished titanium (PT) surfaces. MC3T3‐E1 cells were exposed to a single dose at 2, 4, 6, 8, or 10 Gy using a 60 Co source, with tissue culture polystyrene plates chosen as controls. On all surfaces, irradiation resulted in a dose‐dependent decrease in cellular proliferation. At 4 Gy dose, the cell proliferation of cells decreased by 17.8% on MAO and 18.6% on PT surfaces, respectively, compared with nonirradiated controls. Cells exposed to 8 Gy dose showed significant inhibition in collagen secretion and osteogenesis‐related genes expression (OSX, COL‐Iα1, and OCN). In contrast, irradiation increased cell adhesion to three surfaces dose dependently. It was also demonstrated that cells on MAO surface showed higher adhesion and collagen secretion than on PT surface at different radiation doses. This study revealed the effects of irradiation on osteoblasts in vitro on two titanium surfaces. MAO surface could be used in dental implants in irradiated bone due to enhanced adhesion ability and collagen secretion in osteoblasts. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 9–17, 2013.