Premium
Dual functionalization of titanium with vascular endothelial growth factor and β‐defensin analog for potential application in keratoprosthesis
Author(s) -
Tan Xiao Wei,
Lakshminarayanan R.,
Liu Shou Ping,
Goh Eunice,
Tan Donald,
Beuerman Roger W.,
Mehta Jodhbir S.
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32774
Subject(s) - surface modification , cornea , biomedical engineering , angiogenesis , vascular endothelial growth factor , basic fibroblast growth factor , implant , titanium , materials science , adhesion , wound healing , growth factor , chemistry , biophysics , biochemistry , surgery , medicine , ophthalmology , vegf receptors , cancer research , biology , metallurgy , composite material , receptor
Functionalization of material surfaces can improve their biointegration and bactericidal effect. To expand the biomedical applications of titanium in artificial cornea implantation surgery, titanium alloy substrates were coated with polydopamine and dual bound with recombinant vascular endothelial growth factor (VEGF) and anti‐microbial peptide (AMP), SESB2V. Successful chemical binding was assessed with attenuated total reflectance‐Fourier transform infrared spectroscopy and X‐ray photoelectron spectroscopy. Coating thickness was assessed by atomic force microscopy. Cellular studies revealed that the functionalized substrates displayed the abilities to enhance primary human corneal fibroblast adhesion, proliferation, and viability. Angiogenesis assay with human mesenchymal stem cells was used to verify the biological functions of immobilized VEGF while bactericidal assay was evaluated for the anti‐microbial activities of immobilized SESB2V peptide. We found that the titanium surface that was sequentially functionalized with VEGF and SESB2V had enhanced fibroblast proliferation and anti‐microbial properties. The incorporation of such peptides into an artificial cornea implant is important for implant‐tissue integration and wound healing. This may improve implant integration and reduce the risk of device infection following artificial cornea implantation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.