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Surface modification of polypropylene nonwovens with LDV peptidomimetics and their application in the leukodepletion of blood products
Author(s) -
Gérard Estelle,
Bessy Emilie,
Hénard Grégory,
Verpoort Thierry,
MarchandBrynaert Jacqueline
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32720
Subject(s) - polypropylene , peptidomimetic , surface modification , materials science , adhesion , biomedical engineering , chemical engineering , polymer chemistry , chemistry , biophysics , biochemistry , composite material , peptide , medicine , engineering , biology
Abstract For clinical indications and according to European standards, a depletion of the leukocytes from whole blood has to be realized before transfusion to a patient. In this study, the surface of a layer of meltblown oxygen‐plasma treated polypropylene nonwoven (O 2 ‐PP), making part of the composition of leukodepletion filters, was modified with bioactive molecules to enhance the adhesion of leukocytes. These synthetic small molecules (called peptidomimetics) mimic the “Leu‐Asp‐Val” (LDV) tripeptide sequence recognized by the α 4 β 1 integrin, a receptor expressed on leukocytes. Their activity, as ligands of the α 4 β 1 integrin, was confirmed through cell adhesion assays. The peptidomimetics were covalently immobilized on O 2 ‐PP nonwoven via activation of the hydroxyl‐ and carboxyl‐functions displayed on the polymer surface with trifluoro‐triazine reagent, or were simply deposited on the O 2 ‐PP surface. The treated materials were characterized by X‐ray photoelectron spectroscopy, wettability, and morphological analyses, before and after steam sterilization. Experimental filters composed of 10 layers of O 2 ‐PP nonwovens and a last layer modified with the peptidomimetics were evaluated, using whole blood filtration assays, for their leukodepletion efficiency, the recovery of red cells and platelets and the waste of blood, with an objective to design new filters fulfilling practical and medical criteria. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 1513–1523, 2012.