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Efficient transfer of human adipose‐derived stem cells by chitosan/gelatin blend films
Author(s) -
Cheng NaiChen,
Chang HsuHsien,
Tu YuanKun,
Young TaiHorng
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32706
Subject(s) - gelatin , chitosan , spheroid , ultimate tensile strength , materials science , tissue engineering , adipose tissue , elongation , mesenchymal stem cell , biophysics , adhesion , stem cell , membrane , chemistry , chemical engineering , biomedical engineering , microbiology and biotechnology , in vitro , composite material , biochemistry , medicine , biology , engineering
Adipose‐derived stem cells (ASCs) are a potential source of abundant mesenchymal stem cells and represent a promising cell‐based therapy for tissue damage or degeneration conditions. Previous investigations have demonstrated enhanced therapeutic effects of ASCs in a three‐dimensional spheroid culture formulation. In this study, we hypothesize that a composite membrane made of chitosan/gelatin (C/G) is beneficial to facilitate transfer of human ASCs in spheroids. Increasing chitosan content within the blends enhanced the mechanical properties of the sample, including tensile strength and elongation‐at‐break ratio. Although ASC spheroids developed shortly after seeding on pure chitosan films, increasing gelatin proportion in the C/G blends promoted cell adhesion onto the membranes. We also found that ASCs did not proliferate on chitosan films, but C/G blends of different ratios supported ASC proliferation in the first 4 days of culture. However, ASCs on all C/G blends started to detach from the films to form spheroids after day 4, while ASCs on pure gelatin films remained attached and continued to grow. Gradual gelatin release from the C/G blend films, leading to enriched chitosan content in the blends, probably encouraged ASC detachment and spheroid formation. We placed porous collagen matrix on ASC‐seeded C/G blends to simulate the application of ASC‐seeded C/G films onto injured tissue and found that a C/G film composed of 75% chitosan could facilitate significantly more cell transfer into the overlying collagen sponge. Therefore, a blend film containing 75% chitosan and 25% gelatin showed promising results to serve as a biomaterial for human ASC‐based cell therapy. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B:, 2012.