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Reversal of diabetes by βTC3 cells encapsulated in alginate beads generated by emulsion and internal gelation
Author(s) -
Hoesli Corinne A.,
Kiang Roger L. J.,
Mocinecová Dušana,
Speck Madeleine,
Mošková Daniela Jochec,
DonaldHague Christine,
Lacík Igor,
Kieffer Timothy J.,
Piret James M.
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32667
Subject(s) - emulsion , pullulan , materials science , extrusion , chromatography , biomedical engineering , antibody , chemistry , biophysics , medicine , polysaccharide , biochemistry , immunology , composite material , biology
Encapsulation of insulin‐producing cells in alginate beads could improve the treatment of type 1 diabetes by reducing or eliminating the need for immunosuppression. We have recently adapted an emulsion and internal gelation process to β‐cell encapsulation. This process has the advantages of being well suited for m 3 /h production rates and allowing the use of increased alginate concentrations. Compared with 1.5% alginate beads generated by a standard extrusion process, 5% alginate emulsion‐generated beads demonstrated greater in vitro stability and greater volumetric exclusion of antibody‐sized pullulan. When βTC3 cells were transplanted into streptozotocin‐induced allogeneic diabetic mice, a significant decrease in the blood glucose levels was seen within 2 days with the 5% emulsion‐generated beads but not until >16 days with the 1.5% extrusion‐generated beads. This was correlated with higher cell survival and lower graft‐specific plasma immunoglobulin levels. These results suggest that higher‐concentration alginate beads generated by emulsion and internal gelation have improved graft immunoprotection. The emulsion process is a promising and scalable technology for cellular therapies requiring immune isolation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.