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Formulation and in vitro characterization of PEGylated chitosan and polyethylene imine polymers with thrombospondin‐I gene bearing pDNA
Author(s) -
Mehmet Saka Ongun,
Bozkir Asuman
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32661
Subject(s) - transfection , chitosan , polyethylene glycol , materials science , cytotoxicity , pegylation , gene delivery , polymer , peg ratio , zeta potential , cationic polymerization , conjugated system , in vitro , biophysics , polymer chemistry , nanotechnology , organic chemistry , chemistry , biochemistry , nanoparticle , biology , gene , finance , economics , composite material
An ideal gene carrier is required both in safety and efficiency for transfection. We examined the use of water soluble chitosan and polyethyleneimine as a carrier for anti‐angiogenic protein, TSP‐1 coded, in gene delivery. The aim of this study was to synthesize and characterize polyethylene glycol conjugated cationic polymers to increase anti‐angiogenic gene transfection and reduce possible cytotoxicity. Gel electrophoresis study showed strong DNA binding ability of modified cationic polymers. Also structural properties of pegylated polymers were confirmed by 1 H‐NMR. We investigated in vitro properties of PEG conjugated and coated particles which were observed between 145 and 250 nm with the positive zeta potential value. In addition, the chitosan‐based DNA complexes did not induce remarkable cytotoxicity against MCF‐7 cells. Due to low cytotoxicity, we observed high transfection efficiency at chitosan‐based formulations compared with PEI ones. Although transfection studies carried on in vitro conditions, we measured slight increases at transfection with PEGylation. PEG‐conjugated chitosan formulations can be a promising candidate due to its efficiency in condensing and transfection of pDNA, its low cytotoxicty and comparatively high encapsulation degree. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.