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Surface immobilization of MEPE peptide onto HA/β‐TCP ceramic particles enhances bone regeneration and remodeling
Author(s) -
Acharya Bodhraj,
Chun SoYoung,
Kim ShinYoon,
Moon Cheil,
Shin HongIn,
Park Eui Kyun
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.32648
Subject(s) - peptide , chemistry , regeneration (biology) , osteoblast , materials science , biophysics , microbiology and biotechnology , biochemistry , in vitro , biology
Calcium phosphate ceramics have been widely used as scaffolds for bone regeneration. Here, to improve the osteogenic potential of hydroxyapatite/β‐tricalcium phosphate (HA/β‐TCP) and to apply the bioactive peptide in situ , matrix extracellular phosphoglycoprotein (MEPE) peptide, which has been shown to stimulate osteoblast differentiation, was covalently and directionally immobilized on HA/β‐TCP particles. The free‐hydroxyl groups on the surface of the HA/β‐TCP particles were sequentially conjugated with APTES, PEG‐(SS) 2 , and the synthetic MEPE peptide. Using FTIR and XPS, immobilization of the MEPE peptide on the HA/β‐TCP was confirmed. Implantation of the MEPE peptide‐immobilized HA/β‐TCP into calvarial defect and subsequent analyses using a micro CT and histology showed significant bone regeneration and increased bone area (9.89‐fold) as compared to that of unmodified HA/β‐TCP. Moreover, tartrate‐resistant acid phosphatase‐positive osteoclasts were observed in regenerated bone by the MEPE peptide‐immobilized HA/β‐TCP, indicating that the bones newly formed by the MEPE peptide‐immobilized HA/β‐TCP are actively remodeled by osteoclasts. Therefore, our data demonstrate that MEPE peptide immobilization onto the HA/β‐TCP surface stimulates bone regeneration associated with physiological bone remodeling. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.