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Polymer‐free immobilization of a cyclic RGD peptide on a nitinol stent promotes integrin‐dependent endothelial coverage of strut surfaces
Author(s) -
Joner Michael,
Cheng Qi,
SchönhoferMerl Sabine,
Lopez Monica,
Neubauer Stefanie,
MasMoruno Carlos,
Laufer Burkhardt,
Kolodgie Frank D.,
Kessler Horst,
Virmani Renu
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31988
Subject(s) - peptide , integrin , stent , in vivo , materials science , biocompatibility , biophysics , biomedical engineering , adhesion , cell adhesion , chemistry , biochemistry , receptor , medicine , surgery , biology , composite material , microbiology and biotechnology , metallurgy
This study examined the utility of a stabilized cyclic RGD peptide chemically modified to selectively bind to titanium‐oxide for enhanced biocompatibility of self‐expanding nitinol stents. Endothelial cells express integrin receptors that promote attachment to subendothelial matrix proteins. Integrin binding to arginine‐glycine‐aspartic acid (RGD) peptide derivatives mimic naturally occurring adherent interactions. Irreversible covalent surface coating of conventional nitinol stents with a cyclic RGD (cRGD) peptide highly specific for integrin alpha v beta 3 might foster endothelialization after stent implantation. A selective cRGD peptide was irreversibly immobilized onto titanium oxide‐rich nitinol coupons or self‐expanding stents. Functionality of the engrafted RGD peptide was demonstrated using in vitro endothelial bioassays. A subsequent 7‐day in vivo endothelialization study was performed using cRGD‐coated self‐expanding nitinol stents in rabbits. cRGD peptide coating effectively promoted endothelial cell anchorage, migration, and proliferation confirmed by increased focal adhesions. Proof‐of‐concept studies of rabbit cRGD stent implants showed a significant increase in endothelial coverage above stent struts relative to stents coated with BSA (cRGD = 70.1 ± 21.9 vs. BSA = 49.9 ± 21.8%, p < 0.03). Immobilization of cRGD peptides on strut surfaces represents an innovative strategy to improve endothelialization, which may facilitate vascular healing after stent implantation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.