Premium
Fabrication of chitosan/hydroxylapatite composite rods with a layer‐by‐layer structure for fracture fixation
Author(s) -
Pu XiMing,
Sun ZhenZhen,
Hou ZhenQing,
Yang Yun,
Yao QingQing,
Zhang QiQing
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31961
Subject(s) - composite number , materials science , chitosan , hydroxylapatite , composite material , biocompatibility , chemical engineering , nuclear chemistry , chemistry , metallurgy , biochemistry , engineering , enzyme
A composite rod for fracture fixation using chitosan (CHI)/hydroxylapatite (HA) was prepared by means of in situ precipitation, which had a layer‐by‐layer structure, good mechanical properties, and cell compatibilities. The CHI/HA composite rods were precipitated from the chitosan solution with calcium and phosphorus precursors, followed by treatment with a tripolyphosphate–trisodium phosphate solution (pH >13) to crosslink the CHI and to hydrolyze the calcium phosphates to nanocrystalline HA. The results of FTIR, XRD, and TEM measurements confirmed that HA had been formed within the CHI matrix. The effects of the CHI/HA ratios (20/0, 20/1, 20/2, 20/4, and 20/5, w/w) on the mechanical properties were investigated. At the CHI/HA ratio of 20/4 (w/w), the bending strength and modulus of the rods were 133 MPa and 6.8 GPa, respectively. Pre‐osteoblast MC3T3‐E1 cells were cultured in an extract of the CHI/HA rods (20/4, w/w) to study the cell compatibilities of the composite. The observations indicated that the CHI/HA composite could promote the growth of MC3T3‐E1 cells better than the composite without HA ( p < 0.05). Furthermore, the co‐cultivation of the cells and the CHI/HA composite showed that cells fully spread on the surface of the composite with an obvious cytoskeleton organization, which also revealed that the CHI/HA composite had a good biocompatibility. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 1179–1189, 2012.