Premium
Laser microstructuring of photomodified fluorinated ethylene propylene surface for confined growth of Chinese hamster ovary cells and single cell isolation
Author(s) -
Yakunin S.,
Fahrner M.,
Reisinger B.,
Itani H.,
Romanin C.,
Heitz J.
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31935
Subject(s) - fluoropolymer , materials science , chinese hamster ovary cell , excimer laser , wetting , adhesive , adhesion , fourier transform infrared spectroscopy , x ray photoelectron spectroscopy , laser ablation , laser , cell adhesion , polymer , nanotechnology , chemical engineering , optics , cell culture , composite material , layer (electronics) , physics , engineering , biology , genetics
We present a method for laser lithography of cell‐adhesive arrays on a fluoropolymer surface. The method is based on 172 nm excimer‐lamp photomodification in ammonia atmosphere followed by microstructuring by laser ablation. The improved wettability of the polymer is caused by new chemical groups on the surface after the UV treatment that we proved by Fourier transform infrared spectroscopy and X‐ray photoelectron spectroscopy analyses. The cell adhesion properties of micropatterned structures were tested by cultivation of mammalian cells. We show that single elongated cells can grow confined to lines with sharply defined boundaries of the cell‐covered areas. In preliminary experiments, we also demonstrate that the described technique allows the production of single‐cell arrays with variable cell shape. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.