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Biomimetic mineralization of dentin induced by agarose gel loaded with calcium phosphate
Author(s) -
Ning TianYun,
Xu XiaoHui,
Zhu LiFang,
Zhu XiaoPeng,
Chu Chun Hung,
Liu LaiKui,
Li QuanLi
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31931
Subject(s) - dentin , agarose , dentinal tubule , mineralization (soil science) , remineralisation , phosphate , chemistry , dentin hypersensitivity , calcium , chromatography , materials science , nuclear chemistry , chemical engineering , composite material , inorganic chemistry , biochemistry , organic chemistry , fluoride , nitrogen , engineering
A novel biomimetic mineralization system was designed to induce a layer of hydroxyapatite on a demineralized dentin surface. This system was constructed as follows. A layer of 0.5% agarose gel containing 0.26 M Na 2 HPO 4 was used to cover acid‐etched dentin slices, followed by a layer of agarose gel without phosphate ions. Then a neutral 0.13 M CaCl 2 solution was added onto the ion‐free gel surface. The mineralization system (dentin‐agarose gel containing phosphate ions‐CaCl 2 solution) was kept in a water bath at 37°C, and the gel and CaCl 2 solution were replaced at various intervals. The results showed that the deposited hydroxyapatite crystals densely packed to each other, completely covered the dentin surface, and occluded the dentinal tubules after 10 days of biomimetic mineralization in vitro . Therefore, this method may provide the experimental basis for dentin remineralization and for a new method to treat dentin hypersensitivity and dental caries. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.