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Magnesium used in bioabsorbable stents controls smooth muscle cell proliferation and stimulates endothelial cells in vitro
Author(s) -
Sternberg Katrin,
Gratz Matthias,
Koeck Kathleen,
Mostertz Joerg,
Begunk Robert,
Loebler Marian,
Semmling Beatrice,
Seidlitz Anne,
Hildebrandt Petra,
Homuth Georg,
Grabow Niels,
Tuemmler Conny,
Weitschies Werner,
Schmitz KlausPeter,
Kroemer Heyo K.
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31918
Subject(s) - magnesium , cell growth , extracellular matrix , intracellular , viability assay , endothelial stem cell , smooth muscle , materials science , cell , in vitro , pharmacology , microbiology and biotechnology , biology , endocrinology , biochemistry , metallurgy
Abstract Magnesium‐based bioabsorbable cardiovascular stents have been developed to overcome limitations of permanent metallic stents, such as late stent thrombosis. During stent degradation, endothelial and smooth muscle cells will be exposed to locally high magnesium concentrations with yet unknown physiological consequences. Here, we investigated the effects of elevated magnesium concentrations on human coronary artery endothelial and smooth muscle cell (HCAEC, HCASMC) growth and gene expression. In the course of 24 h after incubation with magnesium chloride solutions (1 or 10 m M ) intracellular magnesium level in HCASMC raised from 0.55 ± 0.25 m M (1 m M ) to 1.38 ± 0.95 m M (10 m M ), while no increase was detected in HCAEC. Accordingly, a DNA microarray‐based study identified 69 magnesium regulated transcripts in HCAEC, but 2172 magnesium regulated transcripts in HCASMC. Notably, a significant regulation of various growth factors and extracellular matrix components was observed. In contrast, viability and proliferation of HCAEC were increased at concentrations of up to 25 m M magnesium chloride, while in HCASMC viability and proliferation appeared to be unaffected. Taken together, our data indicate that magnesium halts smooth muscle cell proliferation and stimulates endothelial cell proliferation, which might translate into a beneficial effect in the setting of stent associated vascular injury. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.