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Exploring cell compatibility of a fibronectin‐functionalized physically crosslinked poly(vinyl alcohol) hydrogel
Author(s) -
Millon Leonardo E.,
Padavan Donna T.,
Hamilton Amanda M.,
Boughner Derek R.,
Wan Wankei
Publication year - 2012
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31860
Subject(s) - vinyl alcohol , surface modification , self healing hydrogels , fibronectin , materials science , adhesion , polymer chemistry , cell adhesion , tissue engineering , biomedical engineering , chemical engineering , cell , chemistry , composite material , polymer , biochemistry , medicine , engineering
Physically crosslinked poly(vinyl alcohol) (PVA) hydrogels prepared using a low‐temperature thermally cycled process have tunable mechanical properties that fall within the range of soft tissues, including cardiovascular tissue. An approach to render it hemocompatible is by endothelization, but its hydrophilic nature is not conducive to cell adhesion and spreading. We investigated the functionalization reaction of this class of PVA hydrogel with fibronectin (FN) for adhesion and spreading of primary porcine radial artery cells and vascular endothelial cells. These are cells relevant to small‐diameter vascular graft development. FN functionalization was achieved using a multistep reaction, but the activation step involving carbonyl diimidazole normally required for chemically crosslinked PVA was found to be unnecessary. The reaction resulted in an increase in the elastic modulus of the PVA hydrogel but is still well within the range of cardiovascular tissue. Confocal microscopy confirmed the adhesion and spreading of both cell types on the PVA–FN surfaces, whereas cells failed to adhere to the PVA control. This is a first step toward an alternative for the realization of a synthetic replacement small‐diameter vascular graft. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.