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An evaluation of BMP‐2 delivery from scaffolds with miniaturized dental implants in a novel rat mandible model
Author(s) -
Wen Bo,
Karl Matthias,
Pendrys David,
Shafer David,
Freilich Martin,
Kuhn Liisa
Publication year - 2011
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31817
Subject(s) - in vivo , scaffold , biomedical engineering , peg ratio , polyethylene glycol , implant , bone morphogenetic protein 2 , in vitro , materials science , dental implant , dentistry , chemistry , surgery , medicine , biochemistry , biology , microbiology and biotechnology , finance , economics
The purpose of this study was three‐fold: (a) to develop a new small animal model to evaluate dental implant systems that recapitulates aspects of the challenging intraoral environment, (b) screen several scaffolds for in vivo bone forming efficacy when used to deliver non‐glycosylated bone morphogenetic protein‐2 (BMP‐2) together with a miniaturized titanium (Ti) dental implant, and (c) identify correlations between in vitro BMP‐2 release rates and in vivo results. The scaffolds tested were: (1) collagen‐hydroxyapatite composite (Col/HA), (2) polyethylene glycol hydrogel (PEG‐hydrogel), and (3) Col/HA infused with PEG‐hydrogel (Col/HA/PEG‐hydrogel). BMP‐2 delivery directly from the Ti implants rather than from the scaffolds was also tested. MicroCT analyses at 4 weeks showed that the maximum volume and height of new bone occurred when BMP‐2 (10 μg) was delivered from the Col/HA/PEG‐hydrogel scaffolds. BMP‐2 delivery from the Ti implant was not as effective as from the scaffolds. While in vitro BMP‐2 release was highest for the PEG‐hydrogel, the scaffold most successful in vivo was the Col/HA/PEG‐hydrogel scaffold because it had the necessary mechanical strength to perform well in the mandibular bone environment. The in vitro release studies suggested a threshold dose of 5 μg which was borne out by the in vivo dose response studies. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.