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MRI and histologic analysis of collagen type II sponge on repairing the cartilage defects of rabbit knee joints
Author(s) -
Chen Honghui,
Yang Xiaohong,
Liao Yingyang,
Zeng Xuwen,
Liang Peihong,
Kang Ning,
Tan Jianrong,
Liang Zhiping
Publication year - 2011
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31762
Subject(s) - cartilage , staining , sponge , type ii collagen , sirius red , chondrocyte , glycosaminoglycan , metachromasia , collagen, type i, alpha 1 , chemistry , anatomy , pathology , type i collagen , safranin , matrix (chemical analysis) , knee joint , medicine , extracellular matrix , biology , surgery , biochemistry , botany , chromatography
There are limited treatment options for cartilage defects in clinical practice because of the lack of suitable biomaterials. Here, we evaluated the effects of collagen type II sponge on the articular cartilage repairing process using a cartilage injury of a rabbit knee joint model. We showed that the home‐made collagen type II sponges appeared to have a suitable pore size of 93.26 ± 38.4 μm for chondrocyte growth. MRI with H&E staining results demonstrated that the effusion absorption in the collagen type II sponge treated group was quicker than that of the control group. Moreover, sporadic cartilage signals first appeared at 6 weeks in the collagen type II sponge treated group. Safranin O staining and immunohistochemical analysis confirmed that the newly formed cartilage expresses glycosaminoglycan and type II collagen matrix. Using Sirius red polarized light staining, we showed that the newly formed cartilage‐like areas from the collagen type II treated group are significantly greater than those of the control group. Taken together, our data demonstrated that the home‐made collagen type II sponge is able to promote cartilage repair in the cartilage injury of a rabbit knee joint model. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.