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Biophysical characterization of ovine forestomach extracellular matrix biomaterials
Author(s) -
Floden Evan W.,
Malak Sharif F. F.,
BasilJones Melissa M.,
Negron Leonardo,
Fisher James N.,
Lun Stan,
Dempsey Sandi G.,
Haverkamp Richard G.,
Ward Brian R.,
May Barnaby C. H.
Publication year - 2011
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31740
Subject(s) - biomaterial , decellularization , materials science , extracellular matrix , biomedical engineering , adhesion , biophysics , chemistry , composite material , nanotechnology , biology , medicine , biochemistry
Ovine forestomach matrix (OFM) is a native and functional decellularized extracellular matrix biomaterial that supports cell adhesion and proliferation and is remodeled during the course of tissue regeneration. Small angle X‐ray scattering demonstrated that OFM retains a native collagen architecture ( d spacing = 63.5 ± 0.2 nm, orientation index = 20°). The biophysical properties of OFM were further defined using ball‐burst, uniaxial and suture retention testing, as well as a quantification of aqueous permeability. OFM biomaterial was relatively strong (yield stress = 10.15 ± 1.81 MPa) and elastic (modulus = 0.044 ± 0.009 GPa). Lamination was used to generate new OFM‐based biomaterials with a range of biophysical properties. The resultant multi‐ply OFM biomaterials had suitable biophysical characteristics for clinical applications where the grafted biomaterial is under load. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.