Premium
Hyaluronic acid hydrogel modified with nogo‐66 receptor antibody and poly‐ L ‐lysine to promote axon regrowth after spinal cord injury
Author(s) -
Wei YueTeng,
He Yu,
Xu ChangLei,
Wang Ying,
Liu BingFang,
Wang XiuMei,
Sun XiaoDan,
Cui FuZhai,
Xu QunYuan
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31689
Subject(s) - self healing hydrogels , hyaluronic acid , axon , chemistry , spinal cord , spinal cord injury , in vivo , glial scar , myelin , regeneration (biology) , microbiology and biotechnology , medicine , anatomy , central nervous system , biology , polymer chemistry , neuroscience
The biomaterials used for central nervous system injury require not only interacting with specific cell adhesion but also specific growth factor receptors to promote nerve regeneration. In this study, hyaluronic acid (HA)‐based hydrogels modified with poly‐ L ‐lysine (PLL) and nogo‐66 receptor antibody (antiNgR) (HA‐PLL/antiNgR) were administered to rats after lateral hemisection of the spinal cord. Anti‐neurofilament positive axons were found to extend into the HA‐PLL/antiNgR hydrogel at 8 weeks after implantation, which shows significant difference compared with HA‐PLL or blank control group. Electron micrographs of implanted hydrogels showed that there were more cells and normal axons with myelin in the HA‐PLL/antiNgR implant than that of HA‐PLL hydrogel. The antiNgR grafted on HA hydrogels could be detected for 8 weeks after transplantation in vivo . All of these properties may facilitate HA‐PLL/antiNgR hydrogels to become a promising scaffold for repairing spinal cord injury. Nevertheless, both two kinds of modified hydrogels (HA‐PLL/antiNgR and HA‐PLL) showed remarkable advantages in supporting angiogenesis, and simultaneously inhibiting the formation of glial scar. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.