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Biofilm formation and composition on different implant materials in vivo
Author(s) -
AlAhmad A.,
WiedmannAlAhmad M.,
Faust J.,
Bächle M.,
Follo M.,
Wolkewitz M.,
Hannig C.,
Hellwig E.,
Carvalho C.,
Kohal R.
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31688
Subject(s) - biofilm , actinomyces naeslundii , veillonella , cubic zirconia , materials science , titanium , scanning electron microscope , fusobacterium nucleatum , chemistry , composite material , nuclear chemistry , dentistry , streptococcus , bacteria , metallurgy , ceramic , biology , medicine , genetics , periodontitis , porphyromonas gingivalis
Abstract Biofilm formation was evaluated on the following titanium and zirconia implants in vivo : machined titanium (Ti‐m), modified titanium (TiUnite ® ), modified zirconia (ZiUnite ® ), machined alumina‐toughened zirconia (ATZ‐m), sandblasted alumina‐toughened zirconia (ATZ‐s), and machined zirconia (TZP‐A‐m). Bovine enamel slabs were used as controls. Surface morphologies were examined by atomic force (AFM) and scanning electron microscopy (SEM). The surface wettability was also determined. Twelve healthy volunteers wore a splint system with the tested materials. After 3 and 5 days the materials were examined by fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). The levels of Streptococcus spp., Veillonella spp., Fusobacteriaum nucleatum , and Actinomyces naeslundii were quantitatively determined. The biofim thickness was found to be between 19.78 and 36.73 μm after 3 days and between 26.11 and 32.43 μm after 5 days. With the exception of Ti‐m the biofilm thickness after 3 days was correlated with surface roughness. In addition to Streptococcus spp. as the main component of the biofilm (11.23–25.30%), F. nucleatum, A. naeslundii , and Veillonella spp. were also detected. No significant differences in biofilm composition on the implant surfaces could be observed. In total, the influence of roughness and material on biofilm formation was compensated by biofilm maturation. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.