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Use of micro‐Raman spectroscopy to investigate hybrid layer quality in demineralized root dentine
Author(s) -
Rolland Sarah L.,
Walls Angus W. G.,
McCabe John F.,
German Matthew J.
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31683
Subject(s) - adhesive , raman spectroscopy , materials science , penetration (warfare) , dentin , composite material , curing (chemistry) , demineralization , remineralisation , root caries , layer (electronics) , dentistry , enamel paint , physics , operations research , optics , engineering , medicine
Treatment of early root caries using resin adhesives to stabilize demineralized dentine and deprive bacteria of nutrients is a recognized and conservative treatment option. Essential to its success is a stable resin‐dentine interface, with effective infiltration of demineralized dentine and adequate resin curing. The objective of this study was therefore to examine degree of cure (DoC) and depth of penetration of dentine adhesives in demineralized dentine using micro‐Raman spectroscopy. Three commercially available adhesives were applied essentially according to manufacturers' instructions to visibly wet demineralized dentine, with two coats of primer to reduce dilution. Specimens were sectioned perpendicular to the bonded interface and micro‐Raman spectroscopy performed on the cross section. Molecules associated with the adhesive were detected to depths greater than 100 μm indicating a thicker hybrid layer than seen in sound dentine. The hybrid layer showed an increase in proportion of aliphatic CC double bonds at the base of the hybrid layer, which may be a consequence of reduced DoC or phase separation. Micro‐Raman spectra suggest deep zones contain an increased concentration of HEMA and a lower concentration of BisGMA, which is likely to be less stable and may contribute to early breakdown of the dentine adhesive interface. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.