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Characterization of micropatterned nanofibrous scaffolds for neural network activity readout for high‐throughput screening
Author(s) -
Wang Lina,
Kisaalita William S.
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31646
Subject(s) - materials science , characterization (materials science) , nanotechnology , mass transport , throughput , high throughput screening , biomedical engineering , nanofiber , computer science , chemistry , engineering , biochemistry , biochemical engineering , telecommunications , wireless
Abstract Micropatterns were fabricated in nanofibrous poly‐ L ‐lactic acid (PLLA) films by laser micromachining and the resulting scaffolds were characterized with respect to architecture, thermal, mechanical, and mass transport properties. Also, human neural stem cells were successfully cultured in these micropatterned nanofibrous scaffolds (MNFSs). The scaffolds were incorporated in high‐density well plates (e.g., 96‐well plates), creating a platform for high‐throughput screening of drugs with physiologically more relevant networked neural cultures. Through mathematical modeling of the transport of model stimulants, the feasibility of stimulating neural networks cultured in MNFSs was demonstrated. More work is needed to establish biological network activity–MNFS architecture relationships. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.