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Protective effect of PEGylation against poly(amidoamine) dendrimer‐induced hemolysis of human red blood cells
Author(s) -
Wang Wei,
Xiong Wei,
Zhu Yanhong,
Xu Huibi,
Yang Xiangliang
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31558
Subject(s) - dendrimer , pegylation , hemolysis , amidoamine , poly(amidoamine) , peg ratio , chemistry , biophysics , lysis , ethylene glycol , polymer chemistry , biochemistry , polyethylene glycol , organic chemistry , immunology , biology , finance , economics
Poly(amidoamine) (PAMAM) dendrimers are widely used in medical applications. However, dendrimers bearing positively charged surface groups are prone to destabilize cell membrane and cause cell lysis. The lytic effect of dendrimers on red blood cells (RBCs) namely hemolysis is extremely dangerous when administered in vivo . To diminish the hematologic toxicity, we modified PAMAM dendrimers with poly(ethylene glycol) (PEG) of three molecular weights (2k, 5k, and 20k). The protective effect of PEGylation against PAMAM dendrimer‐induced hemolysis was studied. RBCs morphology and surface structure were analyzed by optical microscopy (OM) and atomic force microscopy (AFM). The results indicated that PAMAM and PEG‐2k modified dendrimers induced hemolysis at 0.1 and 0.5 mg/mL respectively, whereas PEG‐5k and PEG‐20k modified dendrimers showed no significant difference in hemolysis compared with control even at 5 mg/mL. OM and AFM investigation indicated PAMAM and PEG‐2k modified dendrimers caused RBCs aggregation and lysis. However, no changes were observed in the overall shape of RBCs treated with PEG‐5k and PEG‐20k modified dendrimers. The surface roughness of RBCs treated with PEGylated dendrimers were far lower than that of RBCs treated with PAMAM dendrimers. This study demonstrated that hemocompatibility of PAMAM dendrimers could be greatly enhanced by PEGylation. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010