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Fibrillogenesis in continuously spun synthetic collagen fiber
Author(s) -
Caves Jeffrey M.,
Kumar Vivek A.,
Wen Jing,
Cui Wanxing,
Martinez Adam,
Apkarian Robert,
Coats Julie E.,
Berland Keith,
Chaikof Elliot L.
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31555
Subject(s) - fiber , materials science , ultimate tensile strength , fibrillogenesis , collagen fiber , extrusion , composite material , glutaraldehyde , fibril , biomedical engineering , biomaterial , transmission electron microscopy , scanning electron microscope , elastic fiber , chemistry , nanotechnology , anatomy , chromatography , biochemistry , medicine
The universal structural role of collagen fiber networks has motivated the development of collagen gels, films, coatings, injectables, and other formulations. However, reported synthetic collagen fiber fabrication schemes have either culminated in short, discontinuous fiber segments at unsuitably low production rates, or have incompletely replicated the internal fibrillar structure that dictates fiber mechanical and biological properties. We report a continuous extrusion system with an off‐line phosphate buffer incubation step for the manufacture of synthetic collagen fiber. Fiber with a cross‐section of 53± 14 by 21 ± 3 μm and an ultimate tensile strength of 94 ± 19 MPa was continuously produced at 60 m/hr from an ultrafiltered monomeric collagen solution. The effect of collagen solution concentration, flow rate, and spinneret size on fiber size was investigated. The fiber was further characterized by microdifferential scanning calorimetry, transmission electron microscopy (TEM), second harmonic generation (SHG) analysis, and in a subcutaneous murine implant model. Calorimetry demonstrated stabilization of the collagen triple helical structure, while TEM and SHG revealed a dense, axially aligned D‐periodic fibril structure throughout the fiber cross‐section. Implantation of glutaraldehyde crosslinked and noncrosslinked fiber in the subcutaneous tissue of mice demonstrated limited inflammatory response and biodegradation after a 6‐week implant period. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010