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Carboxymethylation of the fibrillar collagen with respect to formation of hydroxyapatite
Author(s) -
Ehrlich Hermann,
Hanke Thomas,
Simon Paul,
Born René,
Fischer Christiane,
Frolov Andrej,
Langrock Tobias,
Hoffmann Ralf,
Schwarzenbolz Uwe,
Henle Thomas,
Bazhenov Vasily V.,
Worch Hartmut
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31551
Subject(s) - biomineralization , mineralization (soil science) , chemistry , glucuronic acid , macromolecule , mineralized tissues , biophysics , calcium , glycation , fibrillogenesis , fibril , biochemistry , chemical engineering , polysaccharide , organic chemistry , dentin , materials science , receptor , nitrogen , engineering , composite material , biology
Control over crystal growth by acidic matrix macromolecules is an important process in the formation of many mineralized tissues. Highly acidic macromolecules are postulated intermediates in tissue mineralization, because they sequester many calcium ions and occur in high concentrations at mineralizing foci in distantly related organisms. A prerequisite for biomineralization is the ability of cations like calcium to bind to proteins and to result in concert with appropriate anions like phosphates or carbonates in composite materials with bone‐like properties. For this mineralization process the proteins have to be modified with respect to acidification. In this study we modified the protein collagen by carboxymethylation using glucuronic acid. Our experiments showed unambigously, that N ε ‐carboxymethyllysine is the major product of the in vitro nonenzymatic glycation reaction between glucuronic acid and collagen. We hypothesized that the function of biomimetically carboxymethylated collagen is to increase the local concentration of corresponding ions so that a critical nucleus of ions can be formed, leading to the formation of the mineral. Thus, the self‐organization of HAP nanocrystals on and within collagen fibrils was intensified by carboxymethylation. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010