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Healing of bone defects in the goat mandible, using COLLOSS® E and β‐tricalciumphosphate
Author(s) -
Nienhuijs M. E. L.,
Walboomers X. F.,
Briest A.,
Merkx M. A. W.,
Stoelinga P. J. W.,
Jansen J. A.
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31546
Subject(s) - bone formation , histology , extracellular matrix , scaffold , bone morphogenetic protein , anatomy , chemistry , materials science , biomedical engineering , biology , medicine , microbiology and biotechnology , biochemistry , gene
COLLOSS® E, an equine extracellular matrix product containing native transforming growth factor β1 and several bone morphogenetic proteins, has shown osteoinductive properties in ectopic sites. This study was set up to examine its properties in an orthotoptic site in conjunction with a β‐tricalciumphosphate (β‐TCP) scaffolding material. Thirty‐two 17‐mm circular defects in goat mandibles were filled with COLLOSS E, β‐TCP, COLLOSS E + β‐TCP, or left empty. After 9 weeks the results were quantified by micro‐computed tomography and histology. The empty defects contained the highest percentage of new bone (62%). The β‐TCP scaffold resulted in 38% ( p = 0.0029), the mixture of β‐TCP/COLLOSS E resulted in 36% ( p = 0.0057), while the use of COLLOSS E alone resulted in 55% (not significant p = 0.34). These results show that addition of TCP did not result in the expected synergy with regard to the healing of the defect and seemed even to inhibit the healing process. On the other hand, the addition of COLLOSS E induced the formation of small islands of new bone, not connected to the defect edges. This was not observed in the specimens not containing COLLOSS E (4.61% of bone formation centrally in the defect vs. 0.56%; p = 0.042). In conclusion, the results of the present study are somewhat unexpected in that the empty defects showed the most bone ingrowth; however, this ingrowth was always connected to the defect edges. In contrast, the application of COLLOSS E with or without β‐TCP induced bone formation in the center of the defects also. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010