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Can bone healing in distraction osteogenesis be accelerated by local application of IGF‐1 and TGF‐β1?
Author(s) -
Bernstein Anke,
Mayr Hermann O.,
Hube Robert
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31508
Subject(s) - cancellous bone , bone healing , distraction osteogenesis , callus , dentistry , distraction , osteotomy , medicine , chemistry , materials science , biomedical engineering , surgery , biology , genetics , neuroscience
Because complications of distraction osteogenesis are largely related to the long duration of therapy, increasing efforts were reached to shorten treatment by using osteoconductive replacement materials incorporating bioactive molecules such as IGF‐1 andTGF‐β1. The controlled release of IGF‐1 and TGF‐β1 from coated biodegradable poly( D , L ‐lactide) implants could stimulate fracture healing locally. We investigated the effect of locally applied IGF‐1 and TGF‐β1 from IGF‐1/TGF‐β1‐enriched polylactide membranes on fracture healing in a sheep model of delayed callus formation. Twenty‐eight sheep were used for this study. Callus distraction of 1 mm/day by means of a unilateral fixator was continued for 30 days. At the beginning of the subsequent consolidation phase, either growth factors were applied locally or the defect was packed with cancellous bone, or both. The groups treated with growth factors were compared to a control group. The consolidation phase lasted for 60 days and both tibiae were dissected for histological and histomorphometric analyses. This investigation found a reduced absolute callus area in the lengthening zone in all treatment groups. The two treatment groups that received a membrane coated with growth factors showed distinctly higher relative bone areas than the groups treated with an uncoated membrane or packing of the osteotomy defect with cancellous bone. The differences in bone areas were not statistically significant. Application of the growth factors accelerated bone healing and achieved results comparable with those of established treatment methods (packing with autologous cancellous bone). The best results were achieved with a combination of both methods. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2010