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Effect of low level capacitive‐coupled pulsed electric field stimulation on mineral profile of weight‐bearing bones in ovariectomized rats
Author(s) -
Manjhi Jayanand,
Mathur Rashmi,
Behari Jitendra
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31505
Subject(s) - ovariectomized rat , bone mineral , osteoporosis , calcium , materials science , femur , stimulation , tibia , phosphorus , medicine , endocrinology , anatomy , surgery , metallurgy , estrogen
Abstract Mineral content, mineral composition, and crystalline pattern of bone in osteoporosis are different from those of normal individuals. Present management of bone mineral loss is rather unsatisfactory primarily because of socioeconomic factors and untoward effects of the treatment drugs. We report the efficacy of capacitive‐coupled pulsed electric field (CCPEF) to prevent bone loss in an ovariectomized rat model of osteoporosis. One month postsurgery either leg was stimulated with CCPEF, whereas the other leg did not receive any stimulation (sham exposed). The treatment was given in 60 sessions each of 2 h/d (5 days a week). The control group of rats was sham operated. At the end of the observation period, femur and tibia bones were removed. Their bone mineral content (BMC), calcium, phosphorus, and carbon contents were analyzed and bone mineral density (BMD) was calculated. The BMC data were supported by X‐ray diffraction (XRD) method. In sham‐exposed bones, a statistically significant decrease in BMC, BMD, calcium, and phosphorus contents were obtained as compared to the control. Although in CCPEF bones, there was an attenuation of decrement in the noted parameters except phosphorus. XRD pattern supported these observations. The results suggest that chronic, 60 sessions of 2 h/d, 5 d/wk CCPEF (14 MHz with 16 Hz modulation 16 Hz and 10 V peak to peak) is effective in attenuating the ovariectomy‐induced bone mineral loss in rats. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2010