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Cytotoxicity, calcium release, and pH changes generated by novel calcium phosphate cement formulations
Author(s) -
Khashaba Rania M.,
Lockwood Petra E.,
Lewis Jill B.,
Messer Regina L.,
Chutkan Norman B.,
Borke James L.
Publication year - 2010
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31494
Subject(s) - calcium , monocalcium phosphate , dental cement , nuclear chemistry , chemistry , phosphate , cement , polyacrylic acid , materials science , biochemistry , organic chemistry , polymer , composite material , biology , adhesive , layer (electronics) , fishery , fish <actinopterygii> , fish meal
Few published studies describe the biological properties of calcium phosphate cements (CPCs) for dental applications. We measured several biologically relevant properties of 3 CPCs over an extended (8 wk) interval. Monocalcium phosphate, calcium oxide, and synthetic hydroxyapatite were combined with either modified polyacrylic acid, light‐activated modified polyalkenoic acid, or 35% w/w polymethyl vinyl ether maleic acid to obtain Types I, II, and III CPCs, respectively. Set cements were placed in direct contact with L929 fibroblasts for up to 8 weeks. Media Ca +2 and pH were determined by atomic absorption spectroscopy and pH electrode respectively. Cell mitochondrial function was measured by MTT assay. Type I cements suppressed mitochondrial activity > 90% (vs. Teflon controls), but significantly ( p < 0.05) improved to control levels over 8 weeks. Type II cements suppressed mitochondrial activity > 90% at all times. Type III cements elevated mitochondrial activity significantly after 7 wks. The pH profiles approached neutrality by 24 h, and all cements released calcium into the storage medium at all periods (24 h ‐ 8 wk). We concluded that several types of cements had long‐term biological profiles that show promise for dental applications. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010